Application

3T3-L1 DIFFERENTIATION KIT has been used in:Differentiation of 3T3-L1 preadipocytes to adipocytes.Study of obesity, adipogenesis, lipolysis and lipid metabolismDifferentiation of 3T3-L1 preadipocytes to adipocytesStudy of obesity, adipogenesis, lipolysis and lipid metabolism.

Components

Insulin (1.5 mg/ml) 0.6 ml (Catalog Number DIF001A, Green Cap)Differentiation Cocktail, 1000x (lyophilized) 1 vl (Catalog Number DIF001B, Yellow Cap)DMSO (anhydrous) 0.5 ml (Catalog Number DIF001C, Blue Cap)

General description

3T3-L1 cells are derived from mouse 3T3 cells and provide a widely-used fundamental model in the study of adipose physiology and metabolic diseases. They exhibit a fibroblast-like morphology before differentiation but become rounded and accumulate lipid droplets several days after the initiation of differentiation. The accumulated lipid droplets can be visualized by light microscopy. The in vitro differentiated 3T3-L1 adipocytes result in characteristics similar to tissue-derived adipocytes and have been commonly used to study adipogenesis, lipolysis, and metabolic dysfunctions. The 3T3-L1 Differentiation Kit provides enough supplements to make 100 ml of differentiation medium and 600 ml of maintenance medium which is sufficient material for 12 100 mm culture dishes.

Storage and Stability

Store the kit at −20 °C, protected from light.